Coding
off elemen

Part:BBa_K4841005

Designed by: Dai Yuchen   Group: iGEM23_TSBC-SZ   (2023-08-12)


esal

Leads to disruption of EsaRI70V binding and deactivation of the PesaS promoter, integrated into a combinatorial library of promoter and RBS variants to produce a 28-strain series with a range of AHL production rates.
As known as "off element"

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 98
    Illegal NgoMIV site found at 353
    Illegal AgeI site found at 46
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None